Journal: Journal of pharmacological and toxicological methods

Article Title: Simulated Traumatic Brain Injury in In-Vitro Mouse Neuronal and Brain Endothelial Cell Culture Models

doi: 10.1016/j.vascn.2022.107159

Figure Lengend Snippet: List of Used Material

Article Snippet: Mouse Neuronal Cell Culture Celprogen 11003-02 One primary brain cell line we used in the protocol Mouse Neuronal Cell Culture Complete Growth Glucose and Serum Free Media Celprogen M11003-02GF Flushed with hypoxic gas for hypoxic media preparation (MNCC) Mouse Neuronal Cell Culture Complete Growth Media with 10% Fetal Bovine Serum (FBS) Celprogen M11003-02S For maintenance of MNCC, used for normoxic conditions; also referred to as regular/normal media Mouse Neuronal Cell Culture Extracellular Matrix – 96 Black Wall Well Plates (5/pk) Celprogen E1 1003-02-96BW For fluorometric-based assays (cf.

Techniques: Sparging, Cell Culture, Sterility, Cell Counting, Proliferation Assay, End Point Assay, Activity Assay, CyQUANT Assay, Transferring, Light Microscopy, Saline, LDH Cytotoxicity Assay, Membrane

Journal: Journal of pharmacological and toxicological methods

Article Title: Simulated Traumatic Brain Injury in In-Vitro Mouse Neuronal and Brain Endothelial Cell Culture Models

doi: 10.1016/j.vascn.2022.107159

Figure Lengend Snippet: List of Used Material

Article Snippet: Mouse Neuronal Cell Culture Celprogen 11003-02 One primary brain cell line we used in the protocol Mouse Neuronal Cell Culture Complete Growth Glucose and Serum Free Media Celprogen M11003-02GF Flushed with hypoxic gas for hypoxic media preparation (MNCC) Mouse Neuronal Cell Culture Complete Growth Media with 10% Fetal Bovine Serum (FBS) Celprogen M11003-02S For maintenance of MNCC, used for normoxic conditions; also referred to as regular/normal media Mouse Neuronal Cell Culture Extracellular Matrix – 96 Black Wall Well Plates (5/pk) Celprogen E1 1003-02-96BW For fluorometric-based assays (cf.

Techniques: Sparging, Cell Culture, Sterility, Cell Counting, Proliferation Assay, End Point Assay, Activity Assay, CyQUANT Assay, Transferring, Light Microscopy, Saline, LDH Cytotoxicity Assay, Membrane

Journal: Scientific Reports

Article Title: Venom composition and pain-causing toxins of the Australian great carpenter bee Xylocopa aruana

doi: 10.1038/s41598-022-26867-8

Figure Lengend Snippet: Pain-causing effects of X. aruana venom components. ( a,b ) Application of XYTX 1 -Xa1a (10 μM) to DRG cells produced an immediate and sustained, non-cell-specific increase in [Ca 2+ ] i . ( c ) Potency of XYTX 1 -Xa1a and melittin in F11 cells, as monitored by changes in [Ca 2+ ] i . ( d–f ) The increase in [Ca 2+ ] i caused by XYTX 1 -Xa1a was potentiated by the presence of venom PLA 2 (1 µM), but not XYTX 2 -Xa2a (1 μM). ** P < 0.01 (unpaired t -test). ( g,h ) Shallow intraplantar injection of XYTX 1 -Xa1a (200 pmol) caused spontaneous pain behaviours in mice which was potentiated by co-injection of venom PLA 2 (20 pmol), but not XYTX 2 -Xa2a (20 pmol). XYTX 2 -Xa2a (20 pmol) alone does not cause spontaneous pain behaviours while venom PLA 2 does (20 pmol). Data are expressed as mean ± SEM ( n = 3–6). ( i ) Co-injection of XYTX 1 -Xa1a (200 pmol) plus venom PLA 2 (20 pmol) caused paw swelling. Data are expressed as mean ± SEM ( n = 3–6). * P < 0.05; ** P < 0.01; **** P < 0.0001 (one way-ANOVA with Tukey’s multiple comparisons).

Article Snippet: F11 (mouse neuroblastoma \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times $$\end{document} × DRG neuron hybrid; European Collection of Authenticated Cell Cultures) were cultured as previously described .

Techniques: Produced, Injection